Figure 6.

Testing the super-clamp and poor-clamp mutations in vivo. A, Both mutants show a normal Cpx expression pattern, with Cpx co-localized with the neuronal marker HRP. Scale bar: 10 μm. B, C, Recording traces (B) and EPSP frequencies (C) show that spontaneous transmission is significantly increased in the poor-clamp mutant and significantly decreased in the super-clamp mutant; *p < 0.05, ***p < 0.001. D, mEPSP amplitudes are not affected by the mutations. E, F, The mutations do not affect evoked transmission, as evident from representative EPSP traces (E) and average EPSP amplitudes (F). ns - not significant.