Figure 4.

The expression of ERK1/2 was detected in mice induced by E. coli. After 3 hours of bacterial stimulation, liver tissues of WT CD38^−/− and CD38^−/−TLR4^mut mice were taken out to detect the expression of ERK1/2. The liver tissue was stained by immunohistochemistry, and the pictures were obtained by using an Olympus electron microscope (a). The expression of ERK1/2 was detected by Western blot (b), and relative levels of phospho-ERK1/2 to ERK1/2 (c) were analyzed by ImageJ software. Data are presented as means ± standard deviation. Statistical significance was determined by one-way ANOVA (n = 3, ^∗∗p < 0.01).