Figure 6.

MMP3 regulates the abundance of junction proteins in BMVECs. (a–f) The abundance of MMP3, ZO-1, Occludin, VE-cadherin, and Claudin 5 were (a) evaluated via Western blot in WT BMVECs, WT BMVECs that had been treated with MMP3 (WT+MMP3), and MMP3-KO BMVECs (KO). Cofilin abundance was also evaluated to confirm equal loading, and then (b) MMP3, (c) ZO-1, (d) Occludin, (e) VE-cadherin, and (f) Claudin 5 measurements were quantified via normalization to Cofilin. ^∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ^∗∗p < 0.010. (g) WT, WT+MMP3, and MMP3-KO BMVECs were incubated with ZO-1 primary antibodies and fluorescent secondary antibodies (bar = 20 μm), nuclei were counterstained with DAPI, and then (h) ZO-1 abundance was quantified via measurements of fluorescence intensity. ^∗∗∗∗p < 0.0001. Quantified data are summarized for 3 experiments.