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Primer sequences in RT-qPCR.
| Gene | Primer sequence (5′-3′) |
|---|---|
| miR-520a | F: GCCACCACCATCAGCCATAC |
| R: GCACATTACTCTACTCAGAAGGG | |
| RRM2 | F: CCTGACTGTAAATCGTCCAGTGGTA |
| R: AGTTTGGAAGCGACTGTAGGT | |
| U6 | F: CTCGCAGCGCTTCGACA |
| R: ACTTGCGGCTTCACGAATT | |
| GAPDH | F: GATATATGATCAGTCATCTGC |
| R: TTGGATGATCTGGTTTAGCG |
Note: RT-qPCR: reverse transcription quantitative polymerase chain reaction; RRM2: ribonucleotide reductase subunit 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; F: forward; R: reverse.
