Figure 6.
CNGβ1's N-terminal GARP domain localizes to disk incisures within rod outer segments. A, Tangential sections through Xenopus rod outer segments expressing soluble CNGβ1NT and CNGβ1GARP; FLAG-tagged full-length bovine peripherin-2 or untagged full-length CNGβ1. Diagram of transfected constructs are shown above their corresponding panel. Constructs that include N terminal of human CNGβ1 were detected using an anti-GARP antibody. Scale bar: 5 µm in all panels. B, Quantification of plasma membrane intensity (blue line, inset) normalized to incisure intensity (red line, inset) was performed for all the constructs shown in panel A. Protein residing in the plasma membrane yield a value >1, whereas proteins residing in disk incisures yield a value <1. Unpaired Student's t test was performed for full-length CNGβ1 compared with the other three constructs, p < 0.0001.