Table 1.
Oligonucleotide primers to generate and sequence confirm constructs used in this study
| Primer Name | Sequence (from 5′ to 3′) | Usage |
|---|---|---|
| Mcngb1_fwd | ATGTTGGGCTGGGTCCAAAGGG | PCR mouse CNGβ1 from cDNA |
| mCNGb1_rev | TCACTCCGCCCCCTCTTTCTTC | PCR mouse CNGβ1 from cDNA |
| mCNGB1 ssF1 | GCACAGATCCCAGAACCTTG | Sequencing mouse CNGβ1 |
| mCNGB1 ssF2 | GAGCAGGAAGAGGATGAGGA | Sequencing mouse CNGβ1 |
| mCNGB1 ssF3 | TGCAGCAGGAAGAAGCTACC | Sequencing mouse CNGβ1 |
| mCNGB1 ssF4 | GCGGGGACATCATTACAGAC | Sequencing mouse CNGβ1 |
| mCNGB1 ssF5 | GCAGAACCGTGTCAAGACCT | Sequencing mouse CNGβ1 |
| mCNGB1 ssF6 | CCGAAGCTCTTCAATGCTG | Sequencing mouse CNGβ1 |
| mCNGB1 ss7 | GCATCTTGTCCGGAAGTTGT | Sequencing mouse CNGβ1 |
| mCNGB1 ss8 | GAGGGTTGATGCCAAGTTTG | Sequencing mouse CNGβ1 |
| mCNGB1 ss9 | GGGACTCAGCTGGTGACAG | Sequencing mouse CNGβ1 |
| mCNGB1 ss10 | CACCAAACAGCTATCCAGCA | Sequencing mouse CNGβ1 |
| mCNGB1 ss11 | ATTCTGCTCAAGCCACCTGA | Sequencing mouse CNGβ1 |
| AgeI_hCNGB_F1 | CACATAACCGGTATGTTGGGCTGGGTCCAGAGG | Clone untagged human CNGβ1 |
| hCNGB1ssF1 | TCACAGCATCACGGAGGACC | Sequencing human CNGβ1 |
| hCNGB1ssF2 | GTGCAGACCATCAGCATCCT | Sequencing human CNGβ1 |
| hCNGB1ssF3 | TCAGGAGTGCCTGCCACGAA | Sequencing human CNGβ1 |
| hCNGB1ssF4 | GAGGAGCACTATTGCGACAT | Sequencing human CNGβ1 |
| hCNGB1ssF5 | GTGTACAGGGTCATCAGGAC | Sequencing human CNGβ1 |
| hCNGB1ssF6 | GTTAGCAAAGTCGCACTCTT | Sequencing human CNGβ1 |
| hCNGB1ssF7 | GGCAAAAGGCGGCAAACTTG | Sequencing human CNGβ1 |
| hCNGBtmd-F1 | GGATCCATGCTGACCAACCTGATGTATG | Sequencing human CNGβ1 |
| hCNGBtmd-R1 | ACCGGTATCACATCTCTCATCTGTCCGA | Sequencing human CNGβ1 |
| hCNGBntd-F1 | GGATCCATGTTGGGCTGGGTCCAGAG | Sequencing human CNGβ1 |
| hCNGBntd-R1 | GACCGGTATCAGGTTGGTCAGCGGGTCA | Sequencing human CNGβ1 |
| hCNGB1-ssR1 | GTGGAGAGCTCGGTGGAGAC | Sequencing human CNGβ1 |
| hCNGB1-ssR2 | CCAACAGCCAGCAAGCTTAT | Sequencing human CNGβ1 |
| AgeI_EGFP-Fwd | CACATAACCGGTATGGTGAGCAAGGGCGAGGAGCT | Add N-terminal eGFP tag |
| Age_myc-F | CACATAACCGGTATGGAACAAAAACTCATCTCAGA | Add N-terminal myc tag |
| NotI_myc_Rev | GCATTGGCGGCCGCTCACAGATCCTCTTCTGAGATAAGCTT | Add C-terminal myc tag |
| hCNGBtmd-NotI-R1 | AGAGTCGCGGCCGCTTACACATCTCTCATCTGTCCGA | Truncate human CNGβ1 after TMD domain |
| NotI_GARP_Rev | AGAGTCGCGGCCGCTTTATCCTGAACTGGCAGCCTCGG | Truncate human CNGβ1 after GARP domain |
| NotI-hCNGBntd-R | AGAGTCGCGGCCGCTTACAGGTTGGTCAGCGGGTCAATG | Truncate human CNGβ1 after NTD domain |
| hB1343-NotI-R New | ATAGAGTCGCGGCCGCTTTACTTCTCCACAGCTTTGTTCTCTTC | Truncate human CNGβ1 after R domain |
| AgeI_mCNGA1_Fwd | CAATGCACCGGTGCCGCCACCATGAAGACAAATATTATCAATA | PCR mouse CNGα1 from cDNA |
| NotI_mCNGA1_Rev | GCATTGGCGGCCGCTCAGTCCTGTAGAGACTCCGT | PCR mouse CNGα1 from cDNA |
| mCNGA1-ssF1 | TTCCAGAGGACGGAAACAAG | Sequencing mouse CNGα1 |
| mCNGA1-ssR1 | GGTGGCGAAAATTAAAACTCC | Sequencing mouse CNGα1 |
| xR9APtm_Rev | TTACAGAATAGAATAGAGTGTAATAGCTACAAGGGCTGTTCCACATAAAAGCAAGGACACAATTAACTGTCCGTCCAGGTTGGTCAGCGGGTCAATG | Add C-terminal single-pass TM from R9AP |
| hCNGB-AAAA-Fwd | GCCCTCGGTGAGGATCGCTGCGGCT | Mutagenesis primers for 4A mutant |
| hCNGB-AAAA-Rev | CCGGGATCAGGGCCTGCAGCCGCAG | Mutagenesis primers for 4A mutant |
| mB1-535-Not-R | AGAGTCGCGGCCGCTTCAGGGGCCACTATCAGCATCCG | Truncate mouse CNGβ1 after GLU domain |
| mB1-358-Not-R | AGAGTCGCGGCCGCTTCATATCTCCACTATGGCCTCAC | Truncate mouse CNGβ1 after R domain |
| myc-mCNGB1-F | CAAAAACTCATCTCAGAAGAGGATCTGATGTTGGGCTGGGTCCAAAG | Add myc tag to mouse CNGβ1 N terminal |
| myc-hCNGB1-F | CAAAAACTCATCTCAGAAGAGGATCTGTTGGGCTGGGTCCAGAGGGT | Add myc tag to human CNGβ1 N terminal |
| myc-mB1-359-F | CAAAAACTCATCTCAGAAGAGGATCTGCCCAGGGAGCTGACGAAGAT | Add myc tag to mouse CNGβ1 GLU domain |
| myc-hCNGB1-344-F | CAAAAACTCATCTCAGAAGAGGATCTGATGCCCAGAGAGCTGTCC | Add myc tag to human CNGβ1 GLU domain |