Table 2.
Animal testing systems under development
| Organism | Editing events detected | Primary readout | Secondary readout | Editors | PIsa |
|---|---|---|---|---|---|
| Mouse | NHEJ, HDR, off-target cutting | Fluorescent signal in situ | Luciferase | SpyCas9, SauCas9, Cas12a | J. D. Heaney, M. E. Dickinson, W. R. Lagor |
| Mouse | NHEJ, HDR, base editing, PNA | Fluorescent signal in situ | Luciferase, NaI symporter | SpyCas9, SauCas9, Cas12a, Nme2Cas9, CjeCas9, ABE, CBE, PNA | S. A. Murray, C. M. Lutz |
| Pig | NHEJ, HDR | Fluorescent signal | NaI symporter | SpyCas9, SauCas9, Cas12a, ABE | D. F. Carlson; K. D. Wells, R.S. Prather |
| Macaque | NHEJ, HDR, C base editing | Fluorescent signal | Luciferase | SpyCas9, SauCas9, Cas12a, CBE | J. D. Hennebold; A. F. Tarantal, D. J. Segal |
| Marmoset | NHEJ | Akaluciferase | Fluorescence | SpyCas9, SauCas9, Nme2Cas9, Cas12a, ABE | G. Feng; A. F. Tarantal, D. J. Segal |
NaI, sodium iodide.
aThe lead PI of the project is listed first. Additional PIs follow, listed alphabetically by last name. Reporter Development and Testing Center teams are separated by semicolons.