Skip to main content
. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Cancer Res. 2020 Dec 15;81(5):1347–1360. doi: 10.1158/0008-5472.CAN-20-2275

Figure 6: Cellular response to CDK2 inhibitor, PF06873600.

Figure 6:

(A) ENRICHR analysis to display the top gene ontology sets that were significantly suppressed based on RNAseQ analysis on MCF7-WT and MCF7-RB-del cells treated with palbociclib (250 nM) (green) and PF06873600 (200 nM) (blue) up to 48 H. (B) Growth of MCF7-WT and MCF7-RB-del cells treated with different concentrations of PF06873600. Graphs indicate the mean and standard deviation (SD). (C) Growth of HCC1806 cells treated with different concentrations of PF06873600 up to the indicated number of days. Graphs represent mean and SD. (D) BrdU incorporation in MCF7-WT, MCF7-RB-del and MCF7D1/K4 cells treated with different concentrations of PF06873600 up to 72 H. Bars represent mean and SD (t-test). (E) Phalloidin staining on MCF7-WT, MCF7-RB-del and HCC1806 cells following 48 H exposure with PF06873600 (200 nM). DAPI was used to stain for the nuclei. (F) Cell-cycle profile based on PI staining in MCF7-WT and MCF7-EB-del cells following 48 H exposure with PF06873600. Error bars represent mean and SEM from 2 independent experiments. (G) Relative mRNA expression in MCF7-WT and MCF7-RB-del cells treated with palbociclib (250 nM) (green) and PF06873600 (200 nM) (blue) up to 48 H. Mean and SD are shown. (t-test). (H) Growth of 1222 and 226 cells treated with PF06873600 Mean and SD are shown. (I) ENRICHR analysis to display the top gene ontology sets that were significantly suppressed based on RNAseQ analysis on 226 cells treated with palbociclib (250 nM) (green) and PF06873600 (200 nM) (blue) up to 48 H. (J) Mice bearing HCC1806 xenografts were randomized for treatment with Vehicle (n=8), palbociclib (n=4) and PF06873600 (n=6). Data show the mean and SEM (2-way ANOVA). (K) Box plot represents the weights of the tumors that were excised from mice treated with vehicle, palbociclib and PF06873600. Data show the mean and SEM (t-test). (L) H&E staining on tumor tissues excised from 3226 PDX treated with the vehicle, palbociclib and PF06873600 up to 6 days. Representative images are shown (scale bar = 100 μm). (*p< 0.05, **p<0.01, ***p<0.001).