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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Cancer Res. 2020 Dec 29;81(5):1293–1307. doi: 10.1158/0008-5472.CAN-20-1377

Figure 1: Generation and characterization of Plk1 transgenic mice.

Figure 1:

(A) Schematic of Plk1 transgenic mice generation. (B) β-galactosidase staining of WT: wild-type; TA/+: Plk1TA/+; TA/TA: Plk1TA/TA E13.5 mouse embryos. (C) EGFP fluorescence from 1d-old pups of the indicated genotypes. (D-E) qRT-PCR for Plk1 transcripts in PMEFs (D); tissues from transgenic mice (E) of indicated genotypes. Blue and black arrows show primer positions for analysis of the total p1/p2) and endogenous (p3/p4) Plk1 transcript, respectively. (F-G) Western blot (WB) analysis of PMEF cell extracts (F); tissue extracts (G) from transgenic mice. Plk1 and EGFP WB with α-Tubulin loading control. Bottom panel: Plk1 protein quantification of this WB.