Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-PIWIL4 Dilution: 1:200 |
Thermo Fisher Scientific | Cat#: PA5-3144 RRID: AB_2548922 |
| Mouse monoclonal (CloneB56) anti-MKI67 Dilution: 1:200 |
BD Biosciences | Cat#: 556003 RRID: AB_396287 |
| Goat polyclonal anti-DDX4 Dilution: 1:100 |
R&D Systems | Cat#: AF2030 RRID: AB_2277369 |
| Rabbit monoclonal (D73G4) anti-NANOG Dilution: 1:100 |
Cell Signaling Technology | Cat#:4903 RRID: AB_10559205 |
| Mouse monoclonal anti-CYP17A1 Dilution: 1:200 |
Santa Cruz Biotechnology | Cat#: SC-374244 RRID: AB_10988393 |
| Mouse monoclonal (1A12-1D9) anti-GATA3 Dilution: 1:100 |
Thermo Fisher Scientific | Cat#: MA1028 RRID: AB_2536713 |
| Mouse monoclonal (G-4) anti-GATA4 Dilution: 1:100 |
Santa Cruz Biotechnology | Cat#: SC-25310 RRID: AB_627667 |
| Mouse monoclonal anti-DMRT1 Dilution: 1:100 |
Santa Cruz Biotechnology | Cat#: SC-377167 |
| Rabbit polyclonal anti-SOX9 Dilution: 1:200 |
Millipore | Cat#: AB5535 RRID: AB_2239761 |
| AF488 goat-anti mouse IgG2a | Invitrogen | Cat#: A21131 RRID: AB_2535771 |
| AF594 donkey-anti-mouse IgG | Invitrogen | Cat#: A21203 RRID: AB_2535789 |
| AF594 goat-anti-mouse IgG1 | Invitrogen | Cat#: A21125 RRID: AB_2535767 |
| AF594 donkey-anti-rabbit IgG | Jackson ImmunoResearch | Cat#: 711-585-152 RRID: AB_2340621 |
| AF647 donkey-anti-goat IgG | Invitrogen | Cat#: A21447 RRID: AB_2535864 |
| Biological Samples | ||
| Human testis samples from postnatal donors | DonorConnect | NA |
| Human testis samples from embryonic and fetal stages | University of Washington-Birth Defects Research Lab | NA |
| Human testis samples from Jan’s lab | Karolinska Institutet | NA |
| Deposited Data | ||
| Single cell RNA-seq for embryonic and fetal human testes | This paper | GEO: GSE143356 |
| Single cell RNA-seq for postnatal testes | This paper | GEO: GSE161617 |
| Software and Algorithms | ||
| Seurat (2.3.4) | Butler et al., 2018 | https://satijalab.org/seurat/ |
| Monocle (2.10.1) | (Qiu et al., 2017) | http://cole-trapnell-lab.github.io/monocle-release/ |
| GO (David 6.7) | Huang et al., 2009 | https://david-d.ncifcrf.gov |
| Cell Ranger (2.2.0) | NA | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger |
| Cluster 3.0 | NA | http://bonsai.hgc.jp/~mdehoon/software/cluster/software.htm |
| WGCNA (1.68) | (Langfelder and Horvath, 2008) | https://horvath.genetics.ucla.edu/html/CoexpressionNetwork/Rpackages/WGCNA/Tutorials/ |
| Cytoscape (3.7.2) | (Otasek et al., 2019) | https://cytoscape.org |
| Other | ||
| Single cell RNA-seq for infant and adult human testes | Guo et al., 2018 | GEO: GSE120508 |
| Single cell RNA-seq for neonatal human testes | Sohni et al., 2019 | GEO: GSE124263 |