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(A) MEFs of indicated genotypes were treated with 4-OHT for 2 hours. The cells were then harvested, lysed, and immunoprecipitated (IP) for MDM2 and MDMX, and blotted with the indicated proteins. Input represents 5% of total cell lysate utilized for IP. (B) MEFs of indicated genotypes were treated and blotted as in A. (C) MEFs of indicated genotypes were treated and blotted as in A.
