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. Author manuscript; available in PMC: 2021 Aug 15.
Published in final edited form as: Cancer Res. 2020 Dec 15;81(4):1111–1122. doi: 10.1158/0008-5472.CAN-20-2588

Figure 1. MUC1-C induces BRG1 and ARID1A expression.

Figure 1.

A. LNCaP-AI cells stably transduced with a tet-inducible MUC1shRNA were treated with control vehicle or DOX for 7 days. The indicated mRNA levels were analyzed by qRT-PCR. The results (mean±SD of 4–5 determinations) are expressed as relative mRNA levels compared to that in control cells (assigned a value of 1). B. LNCaP-AI cells expressing a control tet-CshRNA or tet-MUC1shRNA were treated with vehicle or DOX for 7 days. Lysates were immunoblotted with antibodies against the indicated proteins. C. LNCaP cells expressing a tet-inducible MUC1-C (tet-MUC1-C) vector were treated with vehicle or DOX for 7 days. Lysates were immunoblotted with antibodies against the indicated proteins. D. DU-145/tet-MUC1shRNA cells were treated with control vehicle or DOX for 7 days. The indicated mRNA levels were analyzed by qRT-PCR. The results (mean±SD of 4–5 determinations) are expressed as relative mRNA levels compared to that in control cells (assigned a value of 1). E. DU-145/tet-CshRNA and DU-145/tet-MUC1shRNA cells were treated with vehicle or DOX for 7 days. Lysates were immunoblotted with antibodies against the indicated proteins. F. Lysates from DU-145/CsgRNA and DU-145/MUC1sgRNA cells were immunoblotted with antibodies against the indicated proteins. G. Lysates from NCI-H660 NEPC cells expressing a CshRNA or MUC1shRNA were immunoblotted with antibodies against the indicated proteins.