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. Author manuscript; available in PMC: 2021 Aug 15.
Published in final edited form as: Cancer Res. 2020 Dec 15;81(4):1111–1122. doi: 10.1158/0008-5472.CAN-20-2588

Figure 5. MUC1-C→E2F signaling induces NOTCH1 expression.

Figure 5.

A and B. RNA-seq was performed in triplicate on LNCaP-AI/tet-MUC1shRNA (A, left), DU-145/tet-MUC1shRNA (A, right) and LNCaP/tet-MUC1-C cells (B) treated with vehicle or DOX for 7 days. The datasets were analyzed using the BENPORATH_ES_1 gene signature. C. DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for NOTCH1 mRNA levels by qRT-PCR. The results (mean±SD of 4 determinations) are expressed as relative mRNA levels compared to that in control cells (assigned a value of 1) (left). Lysates from DU-145/tet-CshRNA and DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins (right). D. Lysates from LNCaP-AI/tet-CshRNA and LNCaP-AI/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. E. Lysates from DU-145/CsgRNA and DU-145/MUC1sgRNA cells were immunoblotted with antibodies against the indicated proteins. F. The indicated cells were left untreated or treated with 5 μM GO-203 for 48 h. Lysates were immunoblotted with antibodies against the indicated proteins. G. Lysates from NCI-H660/CshRNA and NCI-H660/MUC1shRNA cells were immunoblotted with antibodies against the indicated proteins. H. DU-145/CshRNA and DU-145/E2F1shRNA cells were analyzed for NOTCH1 mRNA levels by qRT-PCR. The results (mean±SD of 4 determinations) are expressed as relative mRNA levels compared to that in control cells (assigned a value of 1) (left). Lysates were immunoblotted with antibodies against the indicated proteins (right).