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. 2020 Aug 5;42(2):199–208. doi: 10.1038/s41401-020-0480-9

Fig. 1. Identification of natural xanthone compounds that are regulators of mitophagy.

Fig. 1

a Chemical structure of GeB. b, c GeB promotes the translocation of Parkin. YFP-Parkin HeLa cells were treated with GeB (20 μM) for 4 h. Cells were fixed and analyzed by confocal microscopy. Scale bar, 10 μm. CCCP was used as a positive control. The graph shows the number of cells with puncta (mean ± S.D.); n = 3, *P < 0.05; **P < 0.01. d–g GeB promotes the degradation of mitochondrial proteins. YFP-Parkin HeLa cells were treated with GeB for the indicated times and doses and analyzed by Western blotting. CCCP was used as a positive control to induce mitophagy. The Western blot bands were quantified by ImageJ software; n = 3, *P < 0.05. h–k SH-SY5Y cells were treated with GeB or CCCP for the indicated times and doses and analyzed by Western blotting. CCCP was used as a positive control to induce mitophagy. The Western blot bands were quantified by ImageJ software; n = 3, *P < 0.05.