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. 2020 Aug 5;42(2):199–208. doi: 10.1038/s41401-020-0480-9

Fig. 2. GeB promotes Parkin-dependent mitophagy.

Fig. 2

a, b GeB promotes Parkin translocation to mitochondria. YFP-Parkin HeLa cells were treated with GeB (20 μM) for 4 h. Immunostaining for Tom20 (red) was used to identify mitochondria. Cells were analyzed by confocal microscopy. Scale bar, 10 μm. n = 3, **P < 0.01. c, d GeB induces the colocalization of Parkin with p62. YFP-Parkin HeLa cells were treated with GeB (20 μM) for 4 h. Parkin and endogenous p62 were analyzed by confocal microscopy. Colocalization was quantified by Pearson’s correlation coefficient (PCC); n = 3, **P < 0.01. e, f GeB induces the accumulation of p62 in mitochondria. Mitochondrial fractions and whole-cell lysates were prepared from SH-SY5Y cells treated with GeB (20 μM) for different periods of time as indicated and then analyzed by Western blotting. The Western blot bands were quantified by ImageJ software; n = 3, *P < 0.05, **P < 0.01.