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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Cell Stem Cell. 2021 Feb 3;28(4):685–701.e7. doi: 10.1016/j.stem.2020.12.008

Figure 1. Single-cell RNA sequencing reveals WAT depot-dependent heterogeneity of Pdgfrb-expressing cells.

Figure 1.

(A) MuralChaser mice: a ‘Tet-On’ system allowing for indelible labeling of Pdgfrb-expressing cells. The addition of doxycycline (Dox) leads to Cre expression and CRE-dependent activation of membrane GFP (mGFP) reporter expression.

(B) FACS strategy for the isolation of GFP+ cells from the stromal vascular fraction of inguinal WAT (iWAT) for single-cell RNA sequencing.

(C) UMAP analysis of transcriptional profiles of 8,958 iWAT GFP+ cells and 1,424 gonadal WAT (gWAT) GFP+ cells. Left: distribution of GFP+ cells by adipose depot. Right: Unique cell clusters identified in each depot.

(D) Distribution of Gfp, Pdgfrb, Ly6c1, and Cd9 expression within cell clusters shown in (C).

(E) Expression of indicated gWAT APC markers within cell clusters shown in (C).

(F) Expression of indicated gWAT FIPs markers within cell clusters shown in (C).

For panels D-F, transcript counts represent Log2 of gene expression.