Figure 2. Depot-dependent regulation of fibrogenic and adipogenic gene programs in WAT PDGFRβ+ cells.

(A) mRNA levels of indicated adipogenesis (top)- and fibrosis (bottom) -related genes within freshly isolated gWAT APCs and FIPs from chow-fed mice or aged-matched animals maintained on HFD for 10 weeks.
(B) mRNA levels of indicated adipogenesis (top)- and fibrosis (bottom) -related genes within freshly isolated iWAT DPP4− PDGFRβ+ and DPP4+ PDGFRβ+ cells from chow-fed mice or aged-matched animals maintained on HFD for 10 weeks.
Bars represent mean + s.e.m. * denotes p< 0.05 by two-way ANOVA.
Each sample (n) represents 10,000 freshly isolated cells from 2-3 individual depots. n=6 for HFD FIPs. n=4 for all other subpopulations analyzed.