KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-phospho-AKT (Ser473) antibody | Cell Signaling Technology | Cat# 9271; RRID:AB_329825 |
| Anti-AKT antibody | Cell Signaling Technology | Cat# 2920; RRID:AB_1147620 |
| Anti-HIF1α antibody | Cell Signaling Technology | Cat# 36169 RRID: AB_2799095 |
| Anti-phospho-PPARγ (Ser112) antibody | Millipore | Cat# 04-816 RRID: AB_10563102 |
| Anti-PPARγ antibody | Cell Signaling Technology | Cat# 2443 RRID: AB_823598 |
| Anti-phospho-ERK1/2 (Thr202/Tyr204) antibody | Cell Signaling Technology | Cat# 9101 RRID: AB_331646 |
| Anti-ERK1/2 antibody | Cell Signaling Technology | Cat# 4695 RRID: AB_390779 |
| Anti-β-ACTIN antibody | Sigma | Cat# A1978 RRID: AB_476692 |
| Anti-phospho-PPARγ (Ser273) antibody | Banks et al., 2015 | N/A |
| Anti-phospho-PDGFRα (Tyr742) antibody | Abcam | Cat# ab5452 RRID: AB_304899 |
| Anti-PDGFRα antibody | Cell Signaling Technology | Cat# 3174 RRID: AB_2162345 |
| Anti-phospho-PDGFRβ (Tyr751) antibody | Cell Signaling Technology | Cat# 4549 RRID: AB_1147704 |
| Anti-PDGFRβ antibody | Cell Signaling Technology | Cat# 3169 RRID: AB_2162497 |
| Anti-HIF2α antibody | Novus | Cat# NB100-122 RRID: AB_10002593 |
| Anti-FLAG antibody | Sigma | Cat# F1804 RRID: AB_262044 |
| Anti-β-TUBULIN antibody | Cell Signaling Technology | Cat# 2128 RRID: AB_823664 |
| Anti-PLIN1 antibody | Fitzgerald Industries International | Cat# 20R-PP004 RRID: AB_1288416 |
| Anti-GFP antibody | Abcam | Cat# ab13970 RRID: AB_300798 |
| Anti-CD31 antibody | Abcam | Cat# ab28365 RRID: AB_726365 |
| Anti-CD31 antibody | Millipore | Cat# MAB1398Z RRID: AB_94207 |
| Anti-DPP4 antibody | R&D Systems | Cat# AF954 RRID: AB_355739 |
| Anti-CD142 antibody | Sino Biological | Cat# 50413-R001 RRID: AB_2860267 |
| Alexa Fluor 488 goat anti-chicken secondary antibody | Invitrogen | Cat# A-11039 RRID: AB_142924 |
| Alexa Fluor 647 goat anti-guinea pig secondary antibody | Invitrogen | Cat# A-21450 RRID: AB_141882 |
| Alexa Fluor 594 donkey anti-rabbit secondary antibody | Invitrogen | Cat# A-21207 RRID: AB_141637 |
| Alexa Fluor 488 donkey anti-chicken secondary antibody | Jackson ImmunoResearch Labs | Cat# 703-545-155 RRID: AB_2340375 |
| Alexa Fluor 647 donkey anti-goat secondary antibody | Invitrogen | Cat# A-21447 RRID: AB_141844 |
| Alexa Fluor 594 goat anti- guinea pig secondary antibody | Invitrogen | Cat# A-21113 RRID: AB_2535762 |
| Alexa Fluor 647 donkey anti- rabbit secondary antibody | Invitrogen | Cat# A-32795 RRID: AB_2762835 |
| PerCP/Cyanine5.5 anti-mouse CD45 antibody | Biolegend | Cat# 103132 RRID: AB_893340 |
| PerCP/Cyanine5.5 anti-mouse CD31 antibody | Biolegend | Cat# 102420 RRID: AB_10613644 |
| PE anti-mouse CD140b (PDGFRβ) antibody | Biolegend | Cat# 136006 RRID: AB_1953271 |
| APC anti-mouse Ly6C antibody | Biolegend | Cat# 128016 RRID: AB_1732076 |
| FITC anti-mouse CD9 antibody | Biolegend | Cat# 124808 RRID: AB_1279321 |
| BV421 anti-mouse CD26 (DPP4) antibody | BD Biosciences | Cat# 740021 RRID: AB_2739793 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Imatinib (mesylate) | Cayman Chemical | Cat# 13139 |
| Glucose | Sigma | Cat# G7021 |
| Collagenase D | Roche | Cat# 11088882001 |
| RBC lysis buffer | eBioscience | Cat# 00-4300-54 |
| Lipofectamine LTX | ThermoFisher Scientific | Cat# 15338100 |
| Polybrene | Sigma | Cat# TR-1003 |
| DMEM/F12 GlutaMAX | Life Technologies | Cat# 10565042 |
| DMEM | Cellgro | Cat# 10-014-CV |
| Insulin | Sigma | Cat# I6634 |
| IBMX | Sigma | Cat# I7018 |
| Dexamethasone | Sigma | Cat# D4902 |
| Cobalt chloride | Sigma | Cat# 15862 |
| DMOG | Cayman Chemical | Cat# 71210 |
| Oil Red O | Sigma | Cat# O0625 |
| Dynabeads MyOne Silane beads | ThermoFisher Scientific | Cat# 37002D |
| SPRIselect Reagent beads | Beckman Coulter | Cat# B23317 |
| TRIzol | Invitrogen | Cat# 15596018 |
| Power SYBR Green | Life Technologies | Cat# 4368708 |
| Iamge-iT Fx Signal Enhancer | Invitrogen | Cat# I36933 |
| Prolong Anti-Fade mounting reagent with DAPI | Life Technologies | Cat# P36931 |
| Pierce IP lysis buffer | ThermoFisher Scientific | Cat# 87787 |
| Protease Inhibitor Cocktail | Sigma | Cat# P8340 |
| Phosphatase Inhibitor Cocktail | Sigma | Cat# P5726&P0044 |
| Protein G Sepharose 4 Fast Flow beads | GE Healthcare Bio-sciences | Cat# 17-0618-01 |
| Pierce IgG elution buffer | ThermoFisher Scientific | Cat# 21004 |
| RIPA Lysis Buffer System | Santa Cruz Biotechnology | Cat# sc-24948A |
| PVDF membrane | Millipore | Cat# IPFL00010 |
| Critical Commercial Assays | ||
| Triglyceride determination kit | Sigma | Cat# T2449&F6428 |
| RNeasy Mini Kit | Qiagen | Cat# 5805 |
| RNAqueous micro RNA isolation kit | ThermoFisher Scientific | Cat# AM1931 |
| M-MLV Reverse Transcriptase | Invitrogen | Cat# 28025-013 |
| Deposited Data | ||
| Single cell RNA-seq dataset | This paper | GEO Accession viewer: GSE144090 |
| Mass spectrometry dataset | This paper | MassIVE: MSV000084788 |
| Single cell RNA-seq dataset | Hepler et al., 2018 | GEO Accession viewer: GSE111588 |
| Experimental Models: Cell Lines | ||
| Phoenix cell | ATCC | Cat# CRL-3213 RRID: CVCL_H716 |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6.129-Ppargtm2Rev/J | The Jackson Laboratory | JAX 004584 |
| Mouse: B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J | The Jackson Laboratory | JAX 007676 |
| Mouse: B6.Cg-Tg(tetO-cre)1Jaw/J | The Jackson Laboratory | JAX 006234 |
| Mouse: C57BL/6-Tg(Pdgfrb-rtTA)58Gpta/J | The Jackson Laboratory | JAX 028570 |
| Mouse: TRE-Pparg2 | Shao et al., 2018 | N/A |
| Mouse: TRE-Hif1aDN | Sun et al., 2013 | N/A |
| Oligonucleotides | ||
| A full list of qPCR primers, see Table S4 | This paper | N/A |
| Recombinant DNA | ||
| pMSCV-GFP | Shao et al., 2016 | N/A |
| pMSCV-PPARγ2 | Shao et al., 2016 | N/A |
| pMSCV-PPARγ2 S112A | This paper | N/A |
| pMSCV-PPARγ2 S273A | This paper | N/A |
| psPAX2 | Addgene | Cat# 12260 |
| pMD2.G | Addgene | Cat# 12259 |
| LentiCRISPR V2 | Addgene | Cat# 52961 |
| pUMVC | Addgene | Cat# 8449 |
| pCMV-VSV-G | Addgene | Cat# 8454 |
| Software and Algorithms | ||
| GraphPad Prism | GraphPad Software, Inc | https://www.graphpad.com |
| FlowJo | FlowJo, LLC | https://www.flowjo.com |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| Cell Ranger | 10x Genomics | https://github.com/10XGenomics/cellranger |
| Proteome Discoverer | ThermoFisher Scientific | https://www.thermofisher.com/order/catalog/product/OPTON-30810#/OPTON-30810 |
| Other | ||
| Doxycycline chow diet | Bio-Serv | Cat# S4107 |
| High fat diet | Research Diets | Cat# D12492i |
| Doxycycline high fat diet | Bio-Serv | Cat# S5867 |