Skip to main content
. 2021 Apr 7;12(4):373. doi: 10.1038/s41419-021-03664-1

Fig. 3. Macrophages treated with H-GDEs promote glioma progression in vitro and vivo.

Fig. 3

A Human monocyte U937 cells were incubated with PMA (100 ng/ml) for 24 h in vitro to induce them to differentiate into macrophages. An EdU assay evaluated the proliferation of U87MG or U251 cells cocultured with macrophages treated with PBS, N-GDEs, H-GDEs, or H-GDEs+3-MA, and the results were quantified (scale bar, 100 μm). B The migration capacity of U87MG or U251 cells cocultured with conditioned macrophages was determined. Representative images of migratory cells and quantifications are shown (scale bar, 200 μm). C In vivo bioluminescent imaging analysis of tumor growth in xenograft nude mice bearing U87MG cells with PBS-macrophages, N-GDEs-macrophages, or H-GDEs-macrophages. Representative images on day 5 and 15 post-implantation are shown (data are from five mice per group). D HE staining and IHC staining for Ki-67 of sections from xenograft mouse brains with U87MG and PBS-macrophages, U87MG and N-GDE-macrophages or U87MG and H-GDE-macrophages on the day of euthanasia (scale bar, 200 μm). E Survival analysis of animals implanted with U87MG and PBS-macrophages, U87MG and N-GDEs-macrophages or U87MG and H-GDEs-macrophages (P < 0.01 by log-rank analysis; data from five animals per group). (*P < 0.05; **P < 0.01; ***P < 0.001).