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. 2021 Apr 7;12(4):373. doi: 10.1038/s41419-021-03664-1

Fig. 7. Macrophage overexpression of IL-6 or miR-155-3p promotes glioma progression in vitro and vivo.

Fig. 7

A, C An EdU assay evaluated the proliferation of U87MG or U251 cells cocultured with macrophages transfected with control, IL-6, miR-control, or miR-155-3p. S3I-201 was used to inhibit STAT3 activation. The results were quantified (scale bar, 100 μm). B, D The migration capacity of U87MG or U251 cells cocultured with conditioned macrophages was determined. Representative images of migratory cells and quantifications are shown (scale bar, 200 μm). E In vivo bioluminescent imaging analysis of tumor growth in xenograft nude mice bearing U87MG cells with control-macrophages, IL-6-macrophages, miR-control-macrophages or miR-155-3p-macrophages. Representative images on day 5 and 15 post-implantation are shown (data are from five mice per group). F HE staining and IHC staining for Ki-67 of sections from xenograft mouse brains with U87MG and control-macrophages, U87MG and IL-6-macrophages, U87MG and miR-control-macrophages or U87MG and miR-155-3p-macrophages on the day of euthanasia (scale bar, 200 μm). G Survival analysis of animals implanted with U87MG and control-macrophages, U87MG and IL-6-macrophages, U87MG and miR-control-macrophages or U87MG and miR-155-3p-macrophages (P < 0.05 by log-rank analysis; data from five animals per group). (*P < 0.05; **P < 0.01; ***P < 0.001).