Table 1.
Annealing temperature and qPCR efficiency of 10 candidate reference genes.
| No | Gene symbol | Ta (°C) | Amplicon size (bp) | Efficiency (%) | Correlation coefficient (R2) | Slope |
|---|---|---|---|---|---|---|
| 1 | HPRT1 | 53.0 | 112 | 107.615 | 0.985 | − 3.152 |
| 2 | LDHA | 55.0 | 124 | 100.361 | 0.990 | − 3.313 |
| 3 | SDHA | 55.0 | 159 | 97.513 | 0.998 | − 3.383 |
| 4 | B2M | 56.0 | 175 | 98.149 | 0.990 | − 3.367 |
| 5 | TUBB4 | 57.0 | 208 | 94.980 | 0.982 | − 3.448 |
| 6 | TFRC | 57.0 | 206 | 107.117 | 0.997 | − 3.162 |
| 7 | RPS2 | 58.0 | 224 | 99.499 | 0.994 | − 3.334 |
| 8 | RPL4 | 58.0 | 198 | 95.366 | 0.986 | − 3.438 |
| 9 | ACTB | 58.0 | 92 | 92.845 | 0.997 | − 3.506 |
| 10 | GAPDH | 58.0 | 96 | 99.009 | 0.997 | − 3.346 |
Ta annealing temperature.