Table 2.
Expression stability analysis of each candidate reference gene for the reproductive axis tissues based on ΔCt, geNorm, NormFinder and BestKeeper algorithms.
| Group | Gene symbol | ΔCt | geNorm | NormFinder | Bestkeeper | ||||
|---|---|---|---|---|---|---|---|---|---|
| Rank | ΔCt value | Rank | M value | Rank | SV value | Rank | SD | ||
| ALL | HPRT1 | 3 | 0.043 | 9 | 2.100 | 9 | 1.344 | 4 | 0.941 |
| LDHA | 1 | 0.020 | 7 | 1.586 | 7 | 0.773 | 6 | 1.055 | |
| SDHA | 6 | 0.071 | 5 | 1.436 | 2 | 0.527 | 5 | 0.942 | |
| B2M | 8 | 0.090 | 2 | 1.407 | 3 | 0.538 | 3 | 0.717 | |
| TUBB4 | 9 | 0.091 | 3 | 1.409 | 4 | 0.558 | 7 | 1.290 | |
| TFRC | 2 | 0.040 | 6 | 1.507 | 6 | 0.734 | 2 | 0.459 | |
| RPS2 | 7 | 0.078 | 4 | 1.421 | 5 | 0.578 | 8 | 1.354 | |
| RPL4 | 5 | 0.062 | 8 | 1.684 | 8 | 0.868 | 9 | 1.447 | |
| ACTB | 4 | 0.050 | 1 | 1.304 | 1 | 0.363 | 1 | 0.445 | |
| GAPDH | 10 | 0.228 | 10 | 2.300 | 10 | 1.465 | 10 | 2.098 | |
| H | HPRT1 | 3 | 0.089 | 6 | 0.709 | 7 | 0.312 | 7 | 0.388 |
| LDHA | 4 | 0.100 | 7 | 0.715 | 8 | 0.340 | 2 | 0.211 | |
| SDHA | 1 | 0.070 | 10 | 1.584 | 10 | 1.046 | 10 | 1.354 | |
| B2M | 9 | 0.294 | 4 | 0.632 | 1 | 0.101 | 6 | 0.362 | |
| TUBB4 | 2 | 0.079 | 5 | 0.644 | 5 | 0.222 | 1 | 0.197 | |
| TFRC | 6 | 0.143 | 8 | 0.725 | 6 | 0.305 | 8 | 0.391 | |
| RPS2 | 8 | 0.185 | 3 | 0.623 | 3 | 0.163 | 3 | 0.231 | |
| RPL4 | 7 | 0.184 | 2 | 0.618 | 4 | 0.185 | 4 | 0.303 | |
| ACTB | 5 | 0.128 | 1 | 0.603 | 2 | 0.133 | 5 | 0.316 | |
| GAPDH | 10 | 0.591 | 9 | 1.283 | 9 | 0.752 | 9 | 0.979 | |
| P | HPRT1 | 2 | 0.024 | 1 | 0.288 | 1 | 0.063 | 4 | 0.171 |
| LDHA | 5 | 0.062 | 7 | 0.358 | 7 | 0.163 | 1 | 0.073 | |
| SDHA | 9 | 0.175 | 2 | 0.289 | 2 | 0.065 | 6 | 0.296 | |
| B2M | 6 | 0.116 | 10 | 0.683 | 10 | 0.455 | 8 | 0.423 | |
| TUBB4 | 1 | 0.024 | 5 | 0.337 | 6 | 0.153 | 5 | 0.259 | |
| TFRC | 7 | 0.138 | 4 | 0.323 | 3 | 0.093 | 7 | 0.309 | |
| RPS2 | 3 | 0.043 | 6 | 0.337 | 5 | 0.141 | 2 | 0.144 | |
| RPL4 | 4 | 0.062 | 3 | 0.303 | 4 | 0.098 | 3 | 0.166 | |
| ACTB | 8 | 0.163 | 9 | 0.415 | 9 | 0.251 | 10 | 0.479 | |
| GAPDH | 10 | 0.204 | 8 | 0.401 | 8 | 0.240 | 9 | 0.434 | |
| T | HPRT1 | 1 | 0.009 | 1 | 0.377 | 1 | 0.040 | 1 | 0.461 |
| LDHA | 5 | 0.092 | 2 | 0.406 | 4 | 0.129 | 5 | 0.598 | |
| SDHA | 2 | 0.040 | 4 | 0.426 | 6 | 0.189 | 8 | 0.690 | |
| B2M | 7 | 0.188 | 10 | 1.046 | 10 | 0.705 | 10 | 1.053 | |
| TUBB4 | 9 | 0.201 | 9 | 0.597 | 9 | 0.359 | 3 | 0.495 | |
| TFRC | 10 | 0.209 | 8 | 0.474 | 7 | 0.197 | 7 | 0.625 | |
| RPS2 | 3 | 0.057 | 5 | 0.427 | 5 | 0.160 | 9 | 0.690 | |
| RPL4 | 6 | 0.109 | 3 | 0.411 | 2 | 0.115 | 6 | 0.624 | |
| ACTB | 4 | 0.064 | 6 | 0.431 | 3 | 0.124 | 2 | 0.464 | |
| GAPDH | 8 | 0.191 | 7 | 0.455 | 8 | 0.217 | 4 | 0.560 | |
H hypothalamus, P pituitary, T testis, M Average expression stability, SV Stability Value, SD Standard Deviation. With NormFinder and geNorm, low stability values indicate greater gene expression stability. With BestKeeper, genes with SD values greater than 1 are considered unstable.