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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Mol Cell. 2021 Feb 25;81(7):1384–1396.e6. doi: 10.1016/j.molcel.2021.02.002

Figure 2. Triad residues regulate Gβγ signaling in vivo.

Figure 2.

Wild-type yeast cells transformed with a single copy plasmid containing GPA1 or the indicated mutant G322A (GA), R327S (RS) or E364K (EK).

Left, plate assay with filter disks containing 75, 25, or 8 μg α-factor pheromone.

Right, transcription reporter assay after treatment with the indicated concentration of α factor pheromone. Data are averages of three independent experiments (+/−SE).

Bottom, quantitation of mutant phenotypes. Values indicate extent of growth inhibition, ranging from least (−) to most (+++) turbidity.

See also Figure S2