FIGURE 3.

cox-7C RNAi can only induce the UPR^MITOduring larval stages. (a) Representative images of cox-7C RNAi-induced UPR^MITO in the zcls13[hsp-6p∷GFP] reporter line. The RNAi treatment starts from egg until Day 2 adulthood, and images are taken at Day 2 adulthood. Scale bar, 100 μm. (b) Quantification of imaging experiments in (a). (c–j) A similar set of experiments are conducted with the zcls13[hsp-6p∷GFP] reporter line, and cox-7C RNAi is initiated at different stages: starting from L4 till Day 2 adulthood (c, d), from Day 1 to Day 3 adulthood (e,f), from Day 3 to Day 5 adulthood (g,h), and from Day 8 to Day 10 adulthood (i,j). Images are taken on the last day of RNAi treatment for each condition. The UPR^MITO can be only induced during larval stages but not adulthood. Numbers in parentheses indicate the amount of worms assayed. Data are presented as mean ± SEM, and the statistical significance is assessed using the unpaired student t test. **p < .01; ***p < .001; NS, not significant; RNAi, RNA interference; UPR, unfolded protein response