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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: J Cell Physiol. 2020 Dec 21;236(7):5069–5079. doi: 10.1002/jcp.30215

FIGURE 4.

FIGURE 4

Heat shock induces the UPRCYTO from larval stage untill mid-late life. (a) Representative images of heat shock-induced UPRCYTO in the gpIs1[hsp-16.2p∷GFP] reporter line at the L1 stage. Heat shock (35°C) is applied for 1 h and images are taken 24 h after the heat shock. Scale bar, 100 μm. (b) Quantification of imaging experiments in (a). (c–j) A similar set of experiments are conducted with the gpIs1[hsp-16.2p∷GFP] reporter line at different ages: L4 (c,d), Day 1 adulthood (e,f), Day 4 adulthood (g,h), and Day 9 adulthood (i,j). Heat shock can induce the UPRCYTO at all stages tested, although its inducibility declines during aging. Numbers in parentheses indicate the amount of worms assayed. Data are presented as mean ± SEM, and the statistical significance is assessed using the unpaired student t test. ***p < .001. UPR, unfolded protein response