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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Cancer Lett. 2021 Mar 6;507:1–12. doi: 10.1016/j.canlet.2021.01.028

Fig. 5. Effect of rMMP1 and TGF-β1 on fibroblast senescence and tumor-promoting traits.

Fig. 5.

A, Percentage of SA-βgal+ fibroblasts untreated or treated with 45U/ml rMMP1, 2.5ng/ml TGF-β1 or both for 7 days. Representative images shown at the bottom. Average percentage of SA-βgal+ fibroblasts cocultured with LCC cancer cells was added as a reference (green bars). B-E, Fold cancer cell number density and invasion of H460 (B and D, respectively) and H1299 (C and E, respectively) cells stimulated with the CM of fibroblasts cultured as in (A). Representative images of invading cancer cells are shown above invasion plots. F, TGFB1 mRNA expression from the Sanger dataset in the panel of cancer cell lines used in Fig. 1C. G, Bioactivity of the TGF-β1 within the CM of fibroblasts cocultured with H460 or Bare conditions at day 4. H, Fold COL1A1 mRNA expression in fibroblasts cocultured with H460 cells or Bare as in Fig. 1F. Similar results were obtained with H661 cells (Supplementary Fig. S4). *, P < 0.05; **, P < 0.01; ***, P < 0.005 comparing to either untreated or Bare conditions. #, P < 0.05; ##, P < 0.01; ###, P < 0.005 comparing to coculture with LCC cells. + P < 0.05; ++ P < 0.01; +++ P < 0.005 in all other pairwise comparisons. All comparisons were done using Student t test. Error bars represent mean ± s.e.m. Mean values correspond to n = 2 (G and H) and n = 3 experiments (all other).