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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Drug Alcohol Depend. 2021 Feb 13;221:108598. doi: 10.1016/j.drugalcdep.2021.108598

Fig. 1.

Fig. 1.

Repeated morphine exposure induced hyperalgesia and locomotor hyperactivity. (A) Nociception test. Nociception was tested prior to the final morphine/saline injection and again 40 min after injection. A three-way (drug treatment x sex x pre/post-test) ANOVA followed by a two-way (drug treatment x pre/post-test) ANOVA and Tukey’s multiple comparisons tests revealed hyperalgesia effects prior to injection on Day 10, and a reversal of this effect following morphine treatment (n = 10/group, **** p < 0.0001). (B) Male and female locomotor activity test. A two-way (drug treatment x sex) ANOVA followed by Tukey’s multiple comparisons tests revealed that the morphine group within each sex showed locomotor hyperactivity 1 hr after their final morphine injection, compared to saline controls (n = 5/group; *** p < 0.001, **** p < 0.0001).