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. Author manuscript; available in PMC: 2021 Oct 15.
Published in final edited form as: J Immunol. 2021 Mar 24;206(8):1719–1728. doi: 10.4049/jimmunol.2001266

Figure 3. PPP2R2A deficiency in T cells does not impair T cell development in the thymus and the subset distribution in the spleen.

Figure 3.

(A) Total protein was extracted from thymocytes isolated from R2Afl/fl or LckcreR2Afl/fl mice and subjected to Western blot analysis. (B-D) Thymocytes isolated from R2Afl/fl or LckcreR2Afl/fl mice were stained with CD4 and CD8 antibodies and analyzed by flow cytometry. (B) Representative flow cytometry plots. Cumulative data (n=6 mice/group) depicting the percentages (C) and absolute numbers (D) of thymocyte cell populations. DN: Double negative; DP: Double positive. (E-G) Thymocytes isolated from R2Afl/fl and LckcreR2Afl/fl mice were stimulated with CD3 antibody (1 μg/ml) overnight, and subsequently stained with Annexin V, CD4 and CD8 antibodies followed by FACS analysis. (E and F) Representative flow cytometry plots. (G) Cumulative data (n=6 mice/group) depicting the percentage of Annexin V-positive DN or DP thymocytes. (H-J) Splenocytes isolated from R2Afl/fl or LckcreR2Afl/fl mice were stained with CD3, CD4 and CD8 antibodies and analyzed by flow cytometry. (H) Representative flow cytometry plots. Cumulative data (n = 6 mice/group) depicting the percentages (I) and absolute numbers (J) of splenic CD3, CD4 or CD8 positive cells. All the data were obtained from two independent experiments.