Figure 6. PPP2R2A deficiency in T cells mitigates experimental autoimmune encephalomyelitis (EAE).

EAE was induced in R2A^fl/fl and Lck^creR2A^fl/fl mice by immunization with MOG35-55 emulsified in complete Freund’s adjuvant. (A) Clinical scores. Cumulative results of three independent experiments with total 10 mice per group are shown. Data are shown as Mean ± SEM. P = 0.0187 by two-way ANOVA analysis. (B) Representative H&E staining of spinal cords. Scale bar: 100 μm. (C) Cumulative data elucidating the histopathologic scores for inflammation (n = 5-7 mice/group). FACS analysis of the number of spinal cord-infiltrating (D) CD4 (Thy1.2⁺CD4⁺), (E) CD8 (Thy1.2⁺CD8⁺), (F) Double Negative (DN, Thy1.2⁺CD4⁻CD8⁻) cells, (G-I) IFN-γ- (Thy1.2⁺CD4⁺IFN-γ⁺,Thy1.2⁺CD8⁺IFN-γ⁺; and Thy1.2⁺CD4⁻CD8⁻IFN-γ⁺) and (J-L) IL-17A- (Thy1.2⁺CD4⁺IL-17A⁺, Thy1.2⁺CD4⁻CD8⁻IL-17A⁺ and Thy1.2⁺CD8⁺IL-17A⁺) producing cells. n = 5-6 mice/group. *P<0.05 and **P<0.01 by unpaired T test. The data in (C-I) were obtained from two independent experiments.