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. Author manuscript; available in PMC: 2022 Apr 5.
Published in final edited form as: Dev Cell. 2021 Mar 11;56(7):985–999.e4. doi: 10.1016/j.devcel.2021.02.018

Figure 3. UCP1+ cells are an important source of newly generated beige adipocytes in response to β3 agonist.

Figure 3.

(A) UCP1-DTA (Ucp1-CreERT2; Rosa26RRFP; DTAfl/fl) mouse model.

(B) Experimental procedure. TM-induced 2-month-old UCP1-DTA male mice were treated with vehicle or CL316,243 (β3 agonist) for 7 days before terminal analyses (n=5 mice per group).

(C) Representative H&E staining on IGW sections of mice described in (A). IGW depots were shown at 3 locations: upper, middle, and lower. L: lymph node. Scale = 100 μm.

(D) Representative fluorescence imaging of UCP1 (green) on IGW sections. Scale = 100 μm.

(E) UCP1-PPARγ (Ucp1-CreERT2; PPARγfl/fl) mouse model.

(F) Experimental procedure. TM-induced 2-month-old UCP1-PPARγ male mice were treated with CL316,243 (β3 agonist) for 7 days before terminal analyses (n=5 mice per group).

(G) Representative H&E staining on IGW sections of mice described in (E). Scale = 100 μm.

(H) Representative fluorescence imaging of UCP1 (green) immunostaining on IGW sections. DAPI (blue) stained the nuclei. Scale = 100 μm.