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. 2021 Apr 7;41(4):BSR20203488. doi: 10.1042/BSR20203488

Figure 5. The inhibition of protein synthesis using CHX up-regulates HPV16 E6*I/E7 mRNA levels.

Figure 5

(A) RT-qPCR analysis of SC35 and HPV16 E6all, E6, E6*I and E7 mRNA levels in SiHa cells treated for 6 h with DMSO or CHX at 100 μg/ml. 18S was used for normalization. SC35 was used as a positive control of NMD inhibition. Data are represented as means of three independent experiments. Error bars = s.e.m.; *P<0.05; one-tailed Student’s t test; n.s. non-significant. (B) HPV16 (green) and c-JUN (red) transcripts were detected as punctate dots in DMSO- or CHX-treated SiHa cells using RNAscope and confocal microscopy. Data from one representative experiment of three independent experiments. (C) The number of HPV16 mRNA dots per cell was counted in 50 and 52 cells for DMSO and CHX treated cells, respectively, and are presented as a bar graph. p<0.001; One-tailed Student’s t test.