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. 2021 Mar 25;12:621339. doi: 10.3389/fphar.2021.621339

TABLE 1.

Primer sequences for quantitative real-time PCR amplification.

Gene name Gene number Sequence 5′–3′
GAPDH XM_036165840.1 Forward: 5′-AGG​TCG​GTG​TGA​ACG​GAT​TTG-3′
Reverse: 5′-TGT​AGA​CCA​TGT​AGT​TGA​GGT​CA-3′
AMPK NM_001384157.1 Forward: 5′-GAG​GTT​CAC​AGT​GCC​CTT​CT-3′
Reverse: 5′-TGG​GGT​TTC​ATT​GGA​CTG​CT-3′
SREBP1 XM_036156491.1 Forward: 5′-GCA​GCC​ACC​ATC​TAG​CCT​G-3′
Reverse: 5′-CAG​CAG​TGA​GTC​TGC​CTT​GAT-3′
ACC1 XM_036156218.1 Forward: 5′-ATG​GGC​GGA​ATG​GTC​TCT​TTC-3′
Reverse: 5′-TGG​GGA​CCT​TGT​CTT​CAT​CAT-3′
LXRα XM_006499168.4 Forward: 5′-CTC​AAT​GCC​TGA​TGT​TTC​TCC​T-3′
Reverse: 5′-TCC​AAC​CCT​ATC​CCT​AAA​GCA​A-3′
ABCA1 XM_006537554.2 Forward: 5′-GCT​TGT​TGG​CCT​CAG​TTA​AGG-3′
Reverse: 5′-GTA​GCT​CAG​GCG​TAC​AGA​GAT-3′
SCD1 NM_009127.4 Forward: 5′-TTC​TTG​CGA​TAC​ACT​CTG​GTG​C-3′
Reverse: 5′-CGG​GAT​TGA​ATG​TTC​TTG​TCG​T-3′
FASN XM_030245556.1 Forward: 5′-GGA​GGT​GGT​GAT​AGC​CGG​TAT-3′
Reverse: 5′-TGG​GTA​ATC​CAT​AGA​GCC​CAG-3′