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. 2021 Mar 25;12:621164. doi: 10.3389/fimmu.2021.621164

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Copyright © 2021 Jiang, Lu and Sun

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fever, viremia and neutralizing antibody level of DENV-2 and DENV-3-inoculated tree shrew (n = 9 per group). (A) Schematic diagram of subcutaneous multipoint injection (SMI) and intravenous injection (II) in tree shrew. (B) The fever situations of the entire experimental process. The results from each group were compared using Student’s t test. **P <0.01. (C) The viral load in the serum. The viral RNA copies wERE tested at the day after blood collection on the 2nd, 7th and 15th days post injection. (D) The neutralizing titer in the serum after injection was observed on the 2nd, 7th and 15th days. The results from each group were compared using Student’s t test. *P <0.05, **P <0.01, ***P <0.001.