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Kinetic parameters of M1 sulaftion in human recombinant SULT1A1*1 and M1 glucuronidation in human recombinant UGT1A8.
| Incubation system | SULT1A1*1 | UGT1A8 |
|---|---|---|
| Protein concentration (mg protein/mL) | 0.0250 | 0.100 |
| Incubation time (min) | 15.0 | 60.0 |
| Concentration range (µM) | 0.200–20.0 | 2.00–250 |
| Km (µM) | 3.68 | 63.6 |
| Vmax (pmol·min−1·mg−1 protein) | 44,391 | 548 |
| Vmax/Km (µL·min−1·mg−1 protein) | 12,062 | 8.61 |
