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. 2020 Jul 22;42(2):301–310. doi: 10.1038/s41401-020-0478-3

Fig. 4. The combined treatment impaired mitochondrial functions.

Fig. 4

Huh7 cells were treated as indicated in Fig. 3b. Then, the levels of ROS (a) and mitochondrial-derived ROS (b) were measured with DCFDA and MitoSOX Red, respectively. c The mitochondrial membrane potential was determined with TMRM by using flow cytometry (left panel) and fluorescence microscopy (right panel). d The cellular ATP levels were determined by biochemical assays after the indicated treatments. e Huh7 cells were washed and recultured in fresh XF medium with the indicated drugs for 1 h. Then, the mitochondrial respiratory OCR was determined by Seahorse metabolic assay. Basal respiration OCR, proton leak-involved OCR and ATP-linked OCR are summarized. OCR oxygen consumption rate. **P < 0.01.