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. 2020 Jul 22;42(2):301–310. doi: 10.1038/s41401-020-0478-3

Fig. 5. The combined treatment-induced lysosomal activation and ferritin degradation.

Fig. 5

a Huh7 cells were treated as indicated with or without bafilomycin A1 (100 nM) for 12 h before Western blotting. b Huh7 cells were treated as indicated in (a) for 24 h before the cell survival rate was quantified. c Huh7 cells were treated as indicated in Fig. 4a before lysosomal activity analysis using LysoTracker Red. d, e Huh7 cells were treated as indicated with or without bafilomycin A1 (100 nM) pretreatment for 12 h. Then, the lysosomal enzyme activities of cathepsin L (d) and cathepsin B (e) were quantified. **P < 0.01. ##P < 0.01.