Fig. 2.

The hypomorphic Cd25^Y129H mutation leads to reduced CD25 expression and loss of Foxp3⁺ Treg cells. a Representative FACS plots showing the expression of Foxp3 and CD25 by CD4⁺ T cells in the peripheral lymph nodes (pLNs), spleen, thymus, mesenteric lymph nodes (mLNs), liver, and lungs of 8–12-week-old WT and Cd25^Y129H mice. b Percentages and c absolute cell numbers of Foxp3⁺ Treg cells in different organs of WT (filled circles) and Cd25^Y129H (open circles) mice. Mean fluorescence intensities (MFIs) of CD25 (d) and Foxp3 (e) on Treg cells from WT and Cd25^Y129H mice. Data were derived from at least three experiments with 5–8 mice per genotype and are represented as the mean ± SD. ns not significant; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; two-tailed unpaired Student’s t test