Fig. 5.

Identification of Treg cell subpopulations by single-cell RNA-seq analysis. a Experimental workflow for scRNA-seq analysis including sorting of splenic Foxp3^GFP Treg cells from WT or Cd25^Y129H mice and use of a Chromium Single Cell 3′ Reagent Kit. b UMAP-based clustering of merged scRNA-seq profiles of WT and Cd25^Y129H Treg cells. Heatmap showing the expression of selected marker genes (c) and selected transcription factors (d) for each of the four clusters shown in b. A complete list of the genes and expression values is available in Supplementary Table 3