Fig. 7.
Cd25Y129H mice failed to develop oral tolerance. a Flow chart of tolerance induction protocol. b WT (circles) and Cd25Y129H (squares) mice were fed twice with 25 mg ovalbumin in PBS (OVA; gray bars) or PBS alone (white bars). On day 7, all mice were immunized by subcutaneous injection of OVA emulsified in CFA and challenged 2 weeks later with subcutaneous injection of OVA in PBS into the ear. Tolerance induction was analyzed by measuring ear swelling at 48 h after the challenge (see “Materials and methods”). Data were derived from at least three independent experiments with 6–8 mice per group and are represented as the mean ± SD. ns not significant; *p < 0.05, ***p < 0.001 (two-tailed unpaired Student’s t test)