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. 2020 Jul 9;42(3):436–450. doi: 10.1038/s41401-020-0463-x

Fig. 5. KLF4 transcriptionally regulates the expression of YAP in primary cultured tubular cells.

Fig. 5

a Expression of KLF4 and YAP in primary tubular cells transfected with Ad-Ctrl and Ad-KLF4 for 24 h was assessed by Western blotting. b The mRNA level of YAP was determined by real-time PCR. c Experimental scheme showing luciferase expression construct with the YAP promoter region. Base pair (bp) numbers indicate positions relative to the YAP transcription start site. The blue box indicates the KLF4-binding motif; the genomic sequence of WT pGL3-basic vector is shown below. Mutant YAP promoter construct with a 10-bp deletion (red region) at the pGL3-basic site (mut pGL3-basic). d Luciferase expression analysis based on wild-type and mutant YAP promoter regions in primary tubular cells 1 day after transfection with Ad-Ctrl or Ad-KLF4. Data are presented as the means ± SEM. **P < 0.01 versus the Ad-Ctrl group. e KLF4 binding to the promoter of YAP as determined by chromatin immunoprecipitation assay. The regions with the KLF4-binding sites were quantified and normalized to the levels of the IgG pull-down assay. Data are presented as the means ± SEM. **P < 0.01 compared with IgG control. n = 3 experimental replicates.