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. 2020 Aug 7;42(2):230–241. doi: 10.1038/s41401-020-0490-7

Fig. 7. SIRT3 deficiency promotes NLRP3 activation in the myocardium of the diabetic mice and high-glucose-stimulated cardiomyocytes.

Fig. 7

a–c The protein expression levels of NLRP3, caspase 1, and IL-1β in the myocardium were measured by Western blotting. Significance was determined by one-way ANOVA. Data are presented as the means ± SEM. *P < 0.05, **P < 0.01 vs the control group of the same genotype; #P < 0.05 vs the DM group of WT mice, n = 6. d Mitochondrial NLRP3 was stained using immunofluorescent Alexa Fluor 488 (green)-conjugated IgG and colocalized with MitoTracker red . The nuclei were stained using DAPI (blue). Bar = 25 μm. e NLRP3 and caspase 1 were stained with immunofluorescent Alexa Fluor 488 (green)- and Cy3 (red)-conjugated IgG. The nuclei were stained using DAPI (blue). Bar = 25 μm.