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. 2021 Mar 25;12:630210. doi: 10.3389/fphar.2021.630210

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Copyright © 2021 Zhou, Zou, Zhang, Wang, Yang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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JPYS formula prevents renal cell apoptosis in CKD rats. (A) Terminal dUTP nick end labeling (TUNEL) staining in the kidney (×400, scale bar = 50 μm). TUNEL-positive nuclei are indicated by arrows. (B)The number of TUNEL-positive cells is expressed as the percentage of total cells. (C) Representative western blots for Bax, Bcl-2, and GAPDH protein expression in kidney tissue. (D) Quantitative analysis of Bax/GAPDH. (E) Quantitative analysis of Bcl-2/GAPDH. (F) Quantitative analysis of Bax/Bcl-2. The activity of caspase-3 (G) and caspase-9 (H). All data are expressed as means ± SEM. *p < 0.05, **p < 0.01 vs. the sham group; #p < 0.05, ##p < 0.05 vs. CKD group.