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. 2021 Mar 25;12:624360. doi: 10.3389/fimmu.2021.624360

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Copyright © 2021 Zhang, Liu, Zhong, Zheng, Li, Guo, Zhang, Ran, Zhao, Zhou and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activation and infiltrating of macrophages in the liver tissue of AIH patients. (A–C) Double-immunofluorescence staining for CD68 and MAC387 (A) CD68 and TNF-α (B) CD68 and IL-6 (C) in liver tissues of patients with AIH and hepatic cyst. (D) RT-qPCR analysis of TNF-α, IL-6, IL-1β, CCL2 and CCR2 on tissue homogenates from the liver of the two groups. (E) RT-qPCR analysis of RIP3 and MLKL in the liver of the two groups. (F) Protein levels of RIP3 and MLKL in the liver of the two groups were detected and the relative intensity was quantified. Scale bars: 100μm. The data were presented as means ± SD (Student’s t-test, *p < 0.05, **p < 0.01, ns: p < 0.05).