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. 2021 Mar 25;12:624360. doi: 10.3389/fimmu.2021.624360

Figure 5.

Figure 5

Breakage of intestinal barrier aggravated the activation of RIP3 signaling pathway of the liver tissue. (A) Protein levels of RIP3 and MLKL in the liver of the four groups were detected and the relative intensity was quantified. (B) Representative double-immunofluorescence staining for F4/80 and p-RIP3, F4/80 and p-MLKL of liver tissues in four groups. (C) RT-qPCR analysis of RIP3 and MLKL on liver tissue of DSS-ConA group and GSK872-pretreated group. (D) RT-qPCR analysis of TNF-α, IL-6, and IL-1β on liver tissue of the two groups. (E) RT-qPCR analysis of CCL2 and CCR2 on liver tissue of the two groups. (F) Representative flow cytometry plots and percentage of CD45+F4/80hi CD11blo Kupffer cells and CD45+ F4/80lo CD11bhi infiltrating macrophages in mononuclear cells from liver of the two groups. (G) HE staining of the liver tissue from the two groups. (H) The plasma concentrations of ALT and AST of the two groups. (n=6). Scale bars: 50μm. The data were presented as means ± SD (Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: p < 0.05).