Table 2.
Murine gene | Primer sequences (5′- 3′) |
---|---|
GAPDH | Forward primer: TGTGTCCGTCGTGGATCTGA Reverse primer: CCTGCTTCACCACCTTCTTGA |
ZO-1 | Forward primer: GGGCCATCTCAACTCCTGTA Reverse primer: AGAAGGGCTGACGGGTAAAT |
Occludin | Forward primer: ACTATGCGGAAAGAGTTGACAG Reverse primer: GTCATCCACACTCAAGGTCAG |
TNF-α | Forward primer: ACTCCAGGCGGTGCCTATG Reverse primer: GAGCGTGGTGGCCCCT |
IL-6 | Forward primer: CCAGTTGCCTTCTTGGGACT Reverse primer: GGTCTGTTGGGAGTGGTATCC |
IL-1β | Forward primer: GTGGCTGTGGAGAAGCTGTG Reverse primer: GAAGGTCCACGGGAAAGACAC |
CCL2 | Forward primer: ACCTTTTCCACAACCACCT Reverse primer: GCATCACAGTCCGAGTCA |
CCR2 | Forward primer: AAGGGTCACAGGATTAGGAAG Reverse primer: ATGGTTCAGTCACGGCATA |
RIP3 | Forward primer: GAAGACACGGCACTCCTTGGTA Reverse primer: CTTGAGGCAGTAGTTCTTGGTGG |
MLKL | Forward primer: CCTTGCTTGCTTGCTTTT Reverse primer: TTTCCTTGAGTTTGAGCCA |