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. 2021 Mar 25;12:624360. doi: 10.3389/fimmu.2021.624360

Table 2.

The Oligonucleotide primers used in Realtime-PCR analysis.

Murine gene Primer sequences (5′- 3′)
GAPDH Forward primer: TGTGTCCGTCGTGGATCTGA
Reverse primer: CCTGCTTCACCACCTTCTTGA
ZO-1 Forward primer: GGGCCATCTCAACTCCTGTA
Reverse primer: AGAAGGGCTGACGGGTAAAT
Occludin Forward primer: ACTATGCGGAAAGAGTTGACAG
Reverse primer: GTCATCCACACTCAAGGTCAG
TNF-α Forward primer: ACTCCAGGCGGTGCCTATG
Reverse primer: GAGCGTGGTGGCCCCT
IL-6 Forward primer: CCAGTTGCCTTCTTGGGACT
Reverse primer: GGTCTGTTGGGAGTGGTATCC
IL-1β Forward primer: GTGGCTGTGGAGAAGCTGTG
Reverse primer: GAAGGTCCACGGGAAAGACAC
CCL2 Forward primer: ACCTTTTCCACAACCACCT
Reverse primer: GCATCACAGTCCGAGTCA
CCR2 Forward primer: AAGGGTCACAGGATTAGGAAG
Reverse primer: ATGGTTCAGTCACGGCATA
RIP3 Forward primer: GAAGACACGGCACTCCTTGGTA
Reverse primer: CTTGAGGCAGTAGTTCTTGGTGG
MLKL Forward primer: CCTTGCTTGCTTGCTTTT
Reverse primer: TTTCCTTGAGTTTGAGCCA