Figure 1.

Screening for the response to a preproinsulin truncated peptide library. 5KC T-hybridoma cells expressing ZsGreen-1 upon activation were used to express each TCR clonotype. TCR transductants were cultured with 99 truncated peptide pools containing 12-, 13-, 14-, and 15-mers of peptides ending at the same position of preproinsulin in the presence of autologous EBV-transformed B cells. After overnight culture, cells were evaluated for ZsGreen-1 expression by flow cytometry. Percentages of ZsGreen-1-positive cells in response to each peptide pool are shown for 14 TCR transductants that responded to one or more peptide pools.