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. 2021 Mar 25;12:622647. doi: 10.3389/fendo.2021.622647

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Copyright © 2021 Landry, Anderson, Russ, Yu, Kent, Atkinson, Mathews, Michels and Nakayama

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Preproinsulin-reactive TCR clonotypes restricted by HLA-DP. The TCR 23.G6 transductant was cultured with cognate peptides in the presence of designated antigen presenting cells, followed by assessment of ZsGreen-1 expression by flow cytometry. Left panel: The 23.G6 TCR transductants were cultured with (blue bars) or without (black bars) cognate peptides in the presence of autologous EBV-transformed B cells generated from spleen cells of nPOD 6342, or K562 cells transduced with DR (DRA1*01:01, DRB1*04:01, DRB1*01:01), DQ (DQA1*03:01, DQA1*01:01, DQB1*03:02, DQB1*05:01), or DP (DPA1*01:03, DPB1*04:01) alleles. Culture wells containing an anti-CD3 monoclonal antibody were included as a positive control (red bars). Center panel: The 23.G6 TCR transductant was cultured with (blue bars) or without (black bars) cognate peptides in the presence of K562 cells transduced with a DP gene combination, DPA1*01:03 and DPB1*04:01. Right panel: The 23.G6 TCR transductant was cultured with different concentrations of cognate peptides in the presence of K562 cells transduced with DPA1*01:03 and DPB1*04:01. The experiment in the left panel was performed once. Experiments in the center and right panels were independently repeated three times, and mean values ± standard error of the mean are shown.