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. 2021 Mar 25;12:622647. doi: 10.3389/fendo.2021.622647

Figure 6.

Figure 6

Preproinsulin-reactive TCR clonotypes restricted by HLA-DRB4. (A) The 55.C10 TCR transductant was cultured with (blue bars) or without (black bars) cognate peptides in the presence of autologous EBV-transformed B cells generated from spleen cells of nPOD 6414, or K562 cells transduced with DR (DRA1*01:01, DRB1*03:01, DRB1*09:01), DQ (DQA1*05:01, DQA1*03:03, DQB1*02:01, DQB1*02:02), or DP (DPA1*01:03, DPA1*01:04, DPB1*04:01, DPB1*15:01) alleles. Culture wells containing an anti-CD3 monoclonal antibody were included as a positive control (red bars). (B) The 55.C10 TCR transductant was cultured with (green bars) or without (black bars) cognate peptides in the presence or absence of anti-DR, -DQ, or -DP antibodies. Autologous nPOD 6414 EBV-transformed B cells were used as antigen presenting cells. (C) The 55.C10 TCR transductant was cultured with (blue bars) or without (black bars) cognate peptides in the presence of K562 cells transduced with DRB3 (DRA1*01:01 and DRB3*01:01) or DRB4 (DRA1*01:01 and DRB4*01:01) alleles. (D) The 55.C10 TCR transductant was cultured with different concentrations of cognate peptides in the presence of K562 cells transduced with DRA1*01:01 and DRB4*01:01. Experiment in panels (A, B) were performed once. All remaining experiments in panels (C, D) were independently repeated three times, and mean values ± standard error of the mean are shown.