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Effects of anethole on (A–B) intestinal secretory and (C–F) barrier function of piglets challenged with enterotoxigenic Escherichia coli K88. For PCR assays, n = 8, GAPDH as reference gene; For western blot, n = 3, β-actin as reference protein; The data in each group is expressed as the mean ± SE. Different letters mean statistically significant difference among the groups (P < 0.05).
