Figure 5.

The effect of IRE1α-XBP1 on calcium transfer between the ER and mitochondria in Aβ-treated cells. Cells were pretreated with or without 20 μM 4μ8c for 6 h, followed by treatment with 20 μM Aβ25–35 for 24 h. (A) Basal calcium levels in cells were measured using Fura-2 ratios. (B) Quantification of Tg-induced Ca²⁺ release (reflecting ER Ca²⁺ content). ****p < 0.0001 compared with the control group. ^###P < 0.001 compared with the Aβ25-35-alone group. ^####P < 0.0001 compared with the Aβ25–35-alone group. (C) Cytoplasmic and mitochondrial Ca²⁺ content in cells measured by Fura-2/AM and Rhod-2/AM, respectively. Scale bar = 50 μm. (D) The quantification of cytoplasmic calcium and mitochondrial calcium fluorescence intensity. Red * indicates the comparison of the control group with the Aβ25–35 alone group. Green * indicates the comparison of the Aβ25–35 alone group with the Aβ+4μ8c group. Black * indicates the comparison of the Aβ25–35 alone group with the 2APB+Aβ25–35 group. The data are presented as mean ± SD (n = 3).